The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively.
Jun 1, 2001 Background and Purpose—Ku70 and Ku86, multifunctional DNA repair proteins, bind to broken DNA ends, including double-strand breaks,
Ku70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Ku70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Kontrolluppgiftsblanketter. Det finns ett flertal olika kontrolluppgiftsblanketter i inkomstslaget tjänst: KU10 (lön och förmåner m.m.), KU13 (SINK), KU14 (pensionsgrundande inkomst m.m.), KU16 (sjöinkomst), KU17 (SINK med sjöinkomst), KU18 (pensions- och försäkringsutbetalare) och KU19 (pensions- och försäkringsutbetalare med SINK). Size: 609 amino acids Molecular mass: 69843 Da Quaternary structure: Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex.
(A) The testes of Ku70 2 /and Ku70 2/ Rap1 D mice are of reduced size, as shown for wild-type (left) and Ku70 2/ (right) testes. Scale bar: 5 mm. (B) Ku70 2/ and Ku70 2/ Rap1 D/ (dko) mice display a significant (P,0.001) reduction in testis size compared with wild type (WT) and The DNA end-joining protein Ku70 is one of several proteins that inhibit apoptosis by sequestering the proapoptotic factor Bax from the mitochondria. However, the molecular mechanism underlying Ku70-dependent inhibition of Bax is not fully understood. Here, we show that the absence of Ku70 results in the accumulation of ubiquitylated Bax. Under normal growth conditions, Bax ubiquitylation The protein of 70-kDa molecular weight is a DNA-helicase involved in the DNA double-strand break repair system (Double-Strand Break DNA Repair), more precisely the nonhomologous end joining. Securin 2021-02-22 · Acetylation modulates DNA-mediated Ku70-dependent IFN-λ1 induction. Ku80 colocalizes with Ku70 during Ku70 translocation from the nucleus to the cytoplasm, but not directly involving in Ku70's cytosolic DNA activity.
Securin 2021-02-22 · Acetylation modulates DNA-mediated Ku70-dependent IFN-λ1 induction.
Although the ku70 deletion strain was noted to be more sensitive to UV rays than the corresponding wild-type strain, no lethality, severe growth retardation or loss of gene copy numbers could be detected during repetitive rounds of cultivation and induction of heterologous protein production.
Facebook is showing On the basis of their predicted sequence similarity to human Ku70 and Ku80, cDNAs encoding the first plant homologues of these proteins (AtKu70 and AtKu80, respectively) have now been isolated from Arabidopsis thaliana. AtKu70 and AtKu80 share 28.6 and 22.5% amino acid sequence identity with human Ku70 and Ku80, respectively. Fig. 1. Spermatogenesis in Ku70 and Ku70 Rap1 knockout mice.
Ku70 is a novel Mcl-1 deubiquitinase that could be a potential target for cancer therapy by manipulating Mcl-1 deubiquitination. loss of expression correlates with decreased survival in gall bladder malignancies patients SMAR1-mediated regulation of repair and apoptosis via a complex crosstalk involving Ku70, HDAC6 and Bax.
The Enhanced Cyan Fluorescent Protein (ECFP)-Ku70/EYFP-Ku80 FRET Pair The Ku70/80 heterodimer binds to DNA ends as a starting point for NHEJ complex assembly and juxtaposition Ku70 (E-5): sc-17789 Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com BACKGROUND The Ku protein is localized in the nucleus and is composed of subunits referred to as Ku70 (p70) and Ku86 (p86) which is also known by the synonym Ku80 or (p80). In addition to NHEJ, Ku70/80 also plays an important role in multiple biological processes including telomere maintenance [], HIV replication [] and suppression of apoptosis [].
The stability of these proteins depends on each other. Ku70 is an evolutionarily conserved protein that has functions in DNA repair and maintenance [96]. It is a heterodimeric protein made up of Ku70 and Ku80 [97] . We (unpublished data) and others [43] had evaluated and shown that proteins involved in DNA repair including DNA-PK, p53, ATM and Ku70 had no effect on the type-I-IFN response induced by cytosolic dsDNA stimulation. XRCC6 (X-Ray Repair Cross Complementing 6) is a Protein Coding gene.
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2021-02-26 · Depletion of CBP and/or Ku70 inhibited cell growth and caused cell death. To understand the roles of CBP and Ku70 in human melanoma cells, we designed and synthesized a set of CBP siRNA and Ku70 Although the ku70 deletion strain was noted to be more sensitive to UV rays than the corresponding wild-type strain, no lethality, severe growth retardation or loss of gene copy numbers could be detected during repetitive rounds of cultivation and induction of heterologous protein production. 2021-04-04 · Ku70 A-31G polymorphism is involved in the etiology of RCC; Ku70-1310C/G promoter polymorphism is associated with cancer; Hypoxia-induced cell lethality was facilitated by DNKu70, but substantially repaired upon reoxygenation. Report XRCC6 impacts age-related decline in telomere length but is not associated with longevity. Thus, the Ku70 shRNA cells expressed less Ku70 protein than the Ku70 +/− cells but had a lower, and not the predicted higher, frequency of gene targeting (Tables 1 and 2).
In rice, suppression of either protein has been shown to promote homologous recombination (HR) [13] This effect was exploited to improve gene targeting (GT) efficiency in Arabidopsis thaliana . Ku70 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to Ku70 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
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2001-03-01 · Ku70:Ku80 complex Source: GO_Central "Phylogenetic-based propagation of functional annotations within the Gene Ontology consortium." Gaudet P. , Livstone M.S. , Lewis S.E. , Thomas P.D. Brief Bioinform 12:449-462(2011) [ PubMed ] [ Europe PMC ] [ Abstract ]
2001-03-01 Invitrogen Anti-Ku70 Recombinant Monoclonal (JM61-31), Catalog # MA5-32645. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (Flow) and Immunoprecipitation (IP) applications. This antibody reacts with Human samples. Supplied as 100 µL purified antibody (1 mg/mL). Complex: Ku70:Ku80 complex Macromolecular complex annotations are imported from the Complex Portal.These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background. In vitro, Ku70 -/- mouse fibroblasts displayed an increased rate of sister chromatid exchange and a high frequency of spontaneous neoplastic transformation. In vivo, Ku70 -/- mice, known to be defective in B- but not T-lymphocyte maturation, developed thymic and disseminated T-cell lymphomas at a mean age of 6 months with CD4+/CD8+ tumor cells.
The Ku heterodimer (Ku70 and Ku80 subunits) contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the non-homologous end-joining pathway. The crystal structure of the human Ku heterodimer was determined both alone and bound to a 55-nucleotide DNA element at 2.7 and 2.5 A resolution, respectively.
In this study, CBP was found to positively regulate the expression of Ku70, and both CBP and Ku70 were found to negatively regulate the expression of NOX2, therefore, mitigating the intracellular The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNAdependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70.
2013 Jul 29. This plasmid is available through Addgene. Se hela listan på academic.oup.com of the Ku70/80 heterodimer in vitro. We further defined the function of the Ku70 and Ku80 C-terminal domains in DNA end binding and DNA-PKCS interaction.